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| Fig 1. XYZ view of the final reconstruction. |
A sextuple tilt series of a Flock House Virus specimen was acquired with rotating the sample around the beam axis at 0, 30, 60, 90 and 120 degrees, and collecting for each of those situations a classical single tilt series. For each series, 61 micrographs were taken by moving the sample around the same tilt axis (perpendicular to the optic axis) with a 3 degree increment from -60 to +60 degrees.
This technique allows to reduce quantitatively the reconstruction artifacts associated with the missing wedge problem. At the same time, because six time as much data as in a regular single tilt-series is now available, the background noise becomes also pretty low.
As a drawback of this technique, the sample should be able to withstand more electron beam damage.
When running the TxBR bundle adjustment with a quadratic model (n2=2), the resulting mean reprojection distance error is 0.2 pixel. In figure 2, the error by tilt, by patch and by tilt and patch are displayed.
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Fig 2. Reprojection error result from the bundle adjustment.
On figure 1 and figure 3, views from the 3D reconstruction are shown.
Fig 3. XYZ view of the final reconstruction.