My Protocol for Zenon Kit:
3:1 Fab:Cx43 sigma antibody (0.64 ug/ml)
- Used 1.56 ul of antibody
- Added 20 ul PBS
- Added 5 ul of Zenon A
- Waited for 5 min at RT no lights on
- Added 5 ul of Zenon B and waited for 5 min at RT no lights on
- Used after 10 min
- Put the cover slip on antibody drop and let it for 1 h
- Washed 5 times with PBS
- Fixed a second time in PFA 4% for 15 minutes
- Washed 6 times with PBS.
- Mounted on microscope slide with 70 ul of gelvatol.