Methanol Fixation for Cx43 (to prevent “swiss cheese plaques”)

  1. Plate cells on coverslips
  2. Bring cells to bench
  3. Use -20°C Methanol (100%) – stored in Mothra Freezer
  4. Place Methanol on cells for 30 seconds
  5. Replaced Methanol with 2% Paraformaldehyde (PFA) for 10 minutes
  6. Rinse with 1x PBS 3 times
  7. Continue with labeling protocol of your choosing (straight to gelvatol and mounting on slides if just checking for fluorescence)

    Methanol Fixation for Cx43 Protocol.docx 
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