Welcome to the NCMIR Internal Wiki:
- Public Resources
- General Lab Protocols
- Animal Use
- Bacteria Protein Expression Induction and Purification
- Brain Lysate
- Cell Cycle Synchronization
- DH10 Cells Calcium Chloride Protocol
- DH10 Competent Cells
- Grid Immunolabeling CA
- Immunostaining
- Instructions for Low Dose at the Spirit
- IPTG 1M Aliquots
- Mammalian cell lysates
- Membrane Fixing and Embedding
- Methanol Fixation for Cx43
- MOI Calculation
- Photooxidation
- Pull Down Assay
- Scrape Loading Dye Assay
- SF9 Connexons Purification by Atsu
- SF9 Membrane Preparation CA
- SF9 Transfection Best Procedure
- Staining
- Tips for pure DNA preps
- Tissue Culture
- Ultracryosectioning
- Vacuum Evaporation
- Western Blot
- Ying's protocol for FS epoxy resin
- Zenon Kit CA
- General Lab Protocols
Log in to access private content:
Biological Databases
Equipment Support Roster
Staff/HR Resources
Instrumentation Tutorials
Data Processing Tutorials
Data Workflow
If you need support for any NCMIR system, please try the following:
For self-support info, visit the CRBS Systems Support Wiki.
To create a support (JIRA) ticket, visit https://support.crbs.ucsd.edu or email support@crbs.ucsd.edu.
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